THE USE OF DEOXYCYTIDINE LABELED WITH TRITIUM IN THE 5-POSITION FOR AUTORADIOGRAPHIC STUDY OF LYMPHOCYTES IN VIVO
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概要
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The patterns of labeling of thymic lymphocytes and germinal-center cells of the rat with [5-^3H]deoxycytidine (labeled in the 5-position) in vivo were studied by autoradiographic technique, and compared with the patterns of labeling with [G-^3H]deoxycytidine (generally labeled). With respect to the thymic lymphocytes, no significant difference was observed in labeling intensity between the two labeled markers. In the germinal-center cells, on the other hand, the labeling intensity with [5-^3H]deoxycytidine was found to be significantly weaker than [G-^3H]deoxycytidine labeling. The results are discussed in relation to the ability of these cells to utilize deoxycytidine for the synthesis of DNA-thymine, and the pool size of thymidine within the tissue which suppresses such utilization of deoxycytidine by these cells. It was found that the thymus contained a large pool of thymidine, while in the lymph node the pool seemed much smaller in size.
- 日本組織細胞化学会の論文
著者
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Miyamoto Hajime
Department Of Biopharmaceutics School Of Pharmaceutical Sciences Osaka University
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OSOGOE Bunsuke
Department of Anatomy, Okayama University Medical School
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Osogoe Bunsuke
Department Of Anatomy Kawasaki Medical School
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Miyamoto Hajime
Department Of Anatomy Kyoto University Faculty Of Medicine
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