Changes in the Cyclin D1 Gene Copy Number in Melanoma Cell Lines Detected by Double-Target Fluorescence In Situ Hybridization

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The cyclin D1 gene is often amplified in solid tumors. Six human melanoma cell lines derived from tumors at various stages of progression were analyzed by doble-target fluorescence in situ hybridization (FISH) and dot blot hybridization to detect cyclin D1 gene amplification and deletion. FISH was performed using a probe, for cyclin D1 and an alpha satellite of the chromosome 11 probe, to analyse the cyclin D1 copy number relative to the numbers of chromosome 11 in individual cells. Copies of both the cyclin D1 gene and chromosome 11 were observed in the nuclei of all cell lines. However, the copy numbers of the cyclin D1 gene and of chromosome 11 were closely associated and the copy number ratio of cyclin D1 to chromosome 11 (cylin D1/chromosome 11) was almost one in all cell lines. This finding indicated that the extra copies of cyclin D1 were always accompanied by extra copies of chromosome 11. At the cellular level, cyclin D1 amplification was detected in fewer than 5% of interphase nuclei in only two cell lines, and the gene was deleted in all cell lines to various degrees. Dot blot hybridization revealed no amplification of cyclin D1 in any of the cell lines. We concluded that amplification of the cyclin D1 gene is rare in these melanoma cell lines.
日本組織細胞化学会の論文