Localization of 2, 2, 7-trimethylguanosine, SC-35 and TIAR in Ameloblast Nuclei of the Rat Incisor, and Their Disappearance during Apoptosis
スポンサーリンク
概要
- 論文の詳細を見る
Spliceosomal components, U class snRNA and SC-35, were investigated using anti-2, 2, 7-trimethylguanosine (m3G) antibody for snRNA and anti-SC-35 antibody by immunofluorescence and immunoelectron microscopy in normal and apoptotic ameloblasts. Nuclear substructures, interchromatin granule clusters (IGCs) and perichromatin fibrils (PFs), were positive to anti-m3G antibody and anti-SC-35 antibody. Most of the apoptotic nuclei were not labeled by either antibody. although some were labeled by immunofluorescence microscopy. In earlier apoptotic nuclei, anti-m3G localized in a scattered pattern in the interchromatin space in immunoelectron microscopy. TIAR. a member of the RNA binding proteins with an RNA recognition motif and an effector of apoptosis, localized in the normal ameloblast nucleus, but was mostly lacking in the apoptotic nuclei, excepting occasional diffuse labeling in immunofluorescence microscopy. These results suggest that in addition to nuclear chromatin condensation, rearrangement of interchromatin space including IGCs and PFs occurs during apoptosis, further implying that the modulation of gene transcription and splicing may be accompanied by nuclear changes during the process.
- 日本組織細胞化学会の論文
著者
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Sasaki F
Dept. Of Biology Tsurumi University School Of Dental Medicine
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Sasaki F
Tsurumi Univ.
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Sasaki Fumie
Department Of Biology School Of Dental Medicine Tsurumi University
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Nishikawa Sumio
Department of Biology, Tsurumi University School of Dental Medicine
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Nishikawa Sumio
Department Of Biology Tsurumi Univ. Sch. Of Dental Med.
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Sasaki Fumie
Dept. of Biology, Tsurumi University, School of Dental Medicine
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