ヒト膀胱癌由来培養細胞 (KK-47 細胞) における温熱および放射線による殺細胞効果の研究

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KK-47 cell line, which was established from a human bladder carcinoma in our department in 1977, was used for studies of cell killing effect of heating and/or irradiation. The effect in invitro cultivated KK-47 cells, in Ham's F-12 medium supplemented with 20 per cent calf serum at 37゜C in an atmosphere of 5 per cent CO_2, was determined by a colony-forming method. The doubling time of the cells was 23.5 hours. Using irradiations of 200,400,600, and 800 rads, the radiosensitivities quoted by D_0, Dq, and n.values were 145 rads, 145 rads, and 2.2, respectively. The per cent survivals of the cells exposed for 30 minutes at 40, 41, 42, and 43゜C were 96.6, 93.3, 91.4, and 71.2 per cent, respectively, and those for 2 hours at 40, 41, 42, and 43゜C were 80.6, 75.5, 46.0, and 1.4 per cent, respectively. Exceedingly enhanced cell killing effect was observed at the combinations of 43゜C hyperthermia and the irradiations; heating prior to, posterior to, and through irradiation.
社団法人日本泌尿器科学会の論文