ラットの両側尿管結紮による実験尿毒症腎の電子顕微鏡的研究

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Bilateral ureteral ligation was done on the rats to cause acute uremia, and the dripping fixation method by Maunsbach et al was carried out on thier kidneys. The following results were obtained from light and electron microscopic observations. 1) The penetrating rate of osmium tetroxide by the dripping fixation method in my experiments was 10-15 μ per minute, showing a little lower rate than 20 u per minute reported by Maunsbach et al. 2) The outer cortical tubules fixed by dripping method were not all open lumina against Maunsbach's report and conversly closed lumina were observed in greater number. 3) In the middle portion fixed by both methods of dripping and immersion, remarkable vacuoles appeared in epithelial cells of proximal tubules in both groups of uni and bilateral ureteral ligations and I regard it as an artificial change produced by the fixation. 4) With light microscopy I could not distinguish exactly between vacuoles produced by artificial change and vacuoles of a lysosome like structure, but with electron microscopy the former could be evidently distinguished from the latter. The former were vacuoles produced by separation of plasma membrane. 5) In the group of bilateral ureteral ligation, big vacuoles were observed in the visceral epithelial cells of Bowman's capsule and sometimes these vacuoles and erythrocytes were noted in urinary space of Bowman's capsule. 6) The vacuoles immediately under the microvilli of proximal tubules increased remarkably in the group of bilateral ureteral ligation. 7) In epithelial cells of proximal tubules in the group of bilateral ureteral ligation, swelling of the cytoplasm, projection of whole cells into tubular lumen or occasionally destruction of cells were observed. 8) In epithelial cells of proximal tubules in the group of bilateral ureteral ligation, vacuoles of lysosome-like structure appeared evidently and increased in proportion to the progress of hours after ligation and at last the cells were entirely filled with only these vacuoles. This finding accorded with vacuolar degeneration observed with light microscopy. Similar vacuoles were also noted very slightly in the group of unilateral ureteral ligation. 9) With light microscopy by the Gomori's method, it proved that, acid phosphatase existed in epithelial cells of proximal tubules in the group of bilateral ureteral ligation. Therefore, I regard vacuoles of lysosome-like structure as to be those which lysosome had changed.
社団法人日本泌尿器科学会の論文
1968-11-20

ラットの両側尿管結紮による実験尿毒症腎の電子顕微鏡的研究

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