実験的急性尿細管壊死の組織化学的研究

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The present experiments were carried out with an object to morphologically examine the changes of some enzymes of proximal tubular cells when renal tubular necrosis induced by Mercury, Glycerol and Kanamycin (KM) with 10% Low-Molecular-Dextran (LMWD). The results were summarized as follows: 1) The degeneration and necrosis of proximal tubular cells were observed 24 houres after admini-stration of h/Iercury, Glycerol and KM-LMWD. 2) Three hours after administration of Glycerol, the appearance and formal change of formazan deposits and the decrease of succinic dehydrogenese activity in proximal tubular cells were observed. 3) Three hours after administration of Mercury, histochemical change of succinic dehydrogenese was the same as in Glycerol renal injury, but that of glucose-6-phosphatase was particular evident, so that aggregated droplets which had glucose-6-phosphatase activity appeared in proximal tubular cells. 4) Six hours after administration of KM-LMWD, the decrease of acid-phosphatase locality and the absence of acid-phosphatase activity within vacuoles were observed. The above results indicate that tubular necrosis following Glycerol is mitochondrial origin, tubular necrosis following Mercury is a primary disturbance of microsome and secondary disturbance of mitochondria due to anoxia, and tubular necrosis following KM-LMWD is the release of lysosomal enzyme due to autolysis.
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