口腔癌化学療法に対する実験的研究

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To approximate as nearly as possible to clinical condition for fractionated intra-arterial chemotherapy in oral cancer chemotherapy KB cells of 100x10^4/10ml LY-BSS cultivated at 37℃ for 3 days were exposed for a 30 or 60 minutes period at 37℃ to each of the following antitumor agents such as Nitromin (1mcg/ml, 10mcg/ml), Endoxan (2mcg/ml, 20mcg/ml), Toyomycin (0.01mcg/ml, 0.1mcg/ml), Mitomycin C (0.04mcg/ml, 0.4mcg/ml) and Bleomycin (3mcg/ml, 30mcg/ml) prepared with LY-culture medium containing 10% inactivated bovine serum. The antitumor agents were then washed out with Hanks's BSS. Then new LY-culture medium was added to the cells and kept at 37℃ for 3 days. Then the effects of antitumor agents from standpoint of viable cell number, morphologic changes, succinic dehydrogenase activity were studied. Subculture to another culture flasks was carried out simultaneously. Forgowing operation was repeated 5 times. The results obtained were as follows : 1. The results in inhibition of propagation of cells : Effect of Toyomycin varied with concentration and period of treatment. Nitromin and Mitomycin C had nearly the same tendency in inhibition of propagation in cells but they were affected more by time than concentration of treatment. Endoxan and Bleomycin showed different results from above three agents and it was rather affected by concentration than time of treatment. 2. No significant difference was apparent between any two agents in morphologic changes. However, slight tendency for degeneration of cytoplasm was observed in Nitromin ; regressive changes of nucleolus in Mitomycin C ; multinucleated cells in treatment of 20mcg/ml for 60 minutes in Endoxan, compared with other agents. In addition, segregation, cohesion in chromatin, pycnosis, atrophy, fragmentation as regressive changes in nuclei, atrophy in nucleolus, cytolysis were somewhat more noticeable when propagation of cells was inhibited. 3. Giant cells, microcells were observed in each of experimental series but types of agents, time, concentration and number of treatment did not cause any significant difference. The number of giant cells appeared was higher in lower concentration of agents. Also regressive changes of nuclei in giant cells was stronger in higher concentration of agents. Somewhat more microcells were observed in Bleomycin compared with other agents. 4. Increase and decrease in mitosis nearly agreed with increase and decrease in cells in Nitromin and Toyomycin but did not obtain so close an agreement in Mitomycin C. A lot of mitosis and aberrant division were observed through each of experimental series in Endoxan and Bleomycin compared with other agents. 5. No significant difference in changes of succinic dehydrogenase activity was evident depending on type of agents, time, concentration and number of treatment. The changes, however, had a correlation with increase and decrease in cells.
九州歯科学会の論文
1970-03-31

口腔癌化学療法に対する実験的研究

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