Differential Gene Expression of Matrix Metalloproteinase-3 and -13 during Mineralization of MC3T3-E1 Cells Cultured on Titanium Implant Material
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概要
- 論文の詳細を見る
Successful dental implant requires optimal tissue repair after implant insertion, which involves formation of new bone directly bonded to the device. The objective of this study was to use real-time PCR to investigate the temporal mRNA expression patterns of matrix metalloproteinases (MMP-2, -3, -9, -13, and -14) and tissue inhibitors of metalloproteinases (TIMP-1, -2, and -3) that contribute to tissue repair, during mineralization of MC3T3-E1 cells cultured on titanium (Ti). Lysates of the cells cultured on Ti and plastic wells (Pl) for 10 to 50 days were used for calcium and mRNA quantification. Although the onset of calcium accumulation in the cultures on Ti (30-40 days) was slower than cultures on Pl (20-30 days), the gene expression patterns during mineralization were similar in both cultures. The mRNA expression patterns of MMP-3 and -13 were distinctly different during the period from just before mineralization onset to mineralization development. The level of MMP-13 was substantial until just before the onset of mineralization but declined as mineralization developed, while MMP-3 level increased once mineralization proceeded. MMP-2, -9, and -14 and TIMP-1, -2, and -3 showed constant levels throughout. This report presents the gene expression of MMP-2, -3, -9, -13, and -14 and TIMP-1, -2, and -3 during mineralization by MC3T3-E1 cells cultured on titanium implant material, and suggest temporally distinct roles of MMP-3 and -13 in new bone formation around the implant.
- 硬組織再生生物学会の論文
著者
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SATO Hironobu
Department of Neurosurgery, Kanagawa Childrens Medical Center
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MATSUURA Takashi
Department of Oral Rehabiritation, Fukuoka Denal Collage
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Matsuura Takashi
Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo
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Matsuura Takashi
Department Of Oral Rehabilitation Fukuoka Dental College
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Goto Yousuke
Department of Oral Rehabilitation, Fukuoka Dental College
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Daigo Yuki
Department of Oral Rehabilitation, Fukuoka Dental College
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Daigo Yuki
Department Of Oral Rehabilitation Fukuoka Dental College
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Goto Yousuke
Department Of Oral Rehabilitation Fukuoka Dental College
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Sato Hironobu
Department Of Oral Rehabilitation Fukuoka Dental College
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Sato Hironobu
Department Of Neurosurgery Kanagawa Children's Medical Center
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Sato Hironobu
Department Of Electrical Engineering And Bioscience Waseda University
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Matsuura Takashi
Department Of Cardiovascular Medicine Tottori University Faculty Of Medicine
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Matsuura Takashi
Department of Oral Rehabilitation, Fukuoka Dental College
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- Differential Gene Expression of Matrix Metalloproteinase-3 and -13 during Mineralization of MC3T3-E1 Cells Cultured on Titanium Implant Material〔14巻1号掲載論文の訂正〕
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