Quantitative Analysis of Messenger RNA Expression of Lysyl Hydroxylases in Mandibular and Femoral Bone Marrow of Senescence-accelerated Mice
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概要
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Messenger RNA analysis of bone marrow is a potential examination to diagnose the quality of maxillofacial bone. Lysyl hydroxylases (LHs) are responsible for collagen cross-linking and determine bone quality. To investigate mRNA expression patterns of LHs in bone marrow with potentially different bone quality, we quantified the mRNA expression of LH isoforms (LH1, LH2, and LHS), type I collagen (COLI), and alkaline phosphatase (ALP) in the mandibular and femoral bone marrows of three senescence-accelerated mouse (SAM) strains; the osteoporosis model SAMP6, mandibular osteoarthritis model SAMPS, and their control SAMR1. Total RNAs directly isolated from the bone marrows were used for reverse transcription and the products were then used to quantify mRNA expressions of the molecules using a real-time PCR assay. The expression levels of all the molecules varied in the mandibular and femoral bone marrows. The expression levels of COLI, ALP, and LH2 were higher in the mandibular than in the femoral bone marrow. In the mandibular bone marrow, the expression levels of COLI were significantly different among the three SAM strains. In both bone marrows, an association was observed between COLI and LH1 expressions, and between ALP and LH2 expressions. This study indicates that gene expression patterns of LH1 and LH2 in bone marrows are associated with those of developmental osteoblast markers and that the expression patterns of LH2 and the markers are different in mandibular and femoral bone marrows. Quantitative mRNA analysis of LHs in combination with developmental osteoblast markers in bone marrow may be useful to investigate their relationship with the quality of maxillofacial bone.
- 硬組織再生生物学会の論文
著者
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Matsuura Takashi
Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo
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Matsuura Takashi
Department Of Oral Rehabilitation Fukuoka Dental College
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Sato Hironobu
Department Of Oral Rehabilitation Fukuoka Dental College
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Matsuura Masaro
Department Of Oral Rehabilitation Fukuoka Dental College
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Sato Hironobu
Department Of Electrical Engineering And Bioscience Waseda University
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Yamamoto Katsuki
Department of Oral Rehabilitation, Fukuoka Dental College
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Nagashima Yoshiyuki
Department of Oral Rehabilitation, Fukuoka Dental College
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Nagashima Yoshiyuki
Department Of Oral Rehabilitation Fukuoka Dental College
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Yamamoto Katsuki
Department Of Oral Rehabilitation Fukuoka Dental College
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Matsuura Takashi
Department Of Cardiovascular Medicine Tottori University Faculty Of Medicine
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Matsuura Takashi
Department of Oral Rehabilitation, Fukuoka Dental College
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