磁気により自己発熱する担体を用いた固定化酵素反応の促進

概要

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A magnetic heating method was investigated with the aim of enhancing an immobilized enzyme reaction. A polyacrylamide gel carrier containing an appropriate amount of ferromagnetic powder (57% stainless steel or 45% γ-Fe_2O_3)was found to generate heat in an alternating magnetic field (0-1000 Oe, 0-1.5kHz). In the case of gel containing stainless steel powder, the heat generated(W_<gel> in W or J/s)was given as a function of W_<gel> ∝fH^<1.4>, where f and H are the frequency and magnetic field intensity, respectively. Sucrose hydrolysis was investigated using gel containing ferromagnetic powder, in which invertase was entrapped. Enhancement of the enzyme reaction was proportional to fH^<1.4> in the condition without thermal inactivation of the enzyme. This enhancement effect was due to an increase in the local temperature around the enzyme immobilized within the gel.
公益社団法人日本生物工学会の論文
1998-08-25