光学活性移動相を用いた高速液体クロマトグラフィーによる(S)-(-)-オフロキサシンの光学純度測定(<特集>バイオアナリティカルケミストリー)
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概要
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HPLC using a chiral mobile phase containing either D-or L-phenylalanine-copper (II) complex was evaluated for purity testing of (S) - (-) -ofloxacin (OFLX). As a result, D-phenylalanine was found to be a suitable chiral additive for optical purity testing of (S) - (-) -OFLX. The detection accuracies obtained with a UV detector at 294 nm and a fluorescence detector at Ex. 330, Em. 505 nm, were compared. It was found that the fluorescence detector could detect with greater stability much less (R) - (+) -OFLX in the presence of (S) - (-) -OFLX than the UV detector. (S) - and (R) -OFLXs in spiked serum were determined simultaneously by connecting a UV detector and a fluorescence detector in series.
- 社団法人日本分析化学会の論文
- 1989-11-05
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