電気化学検出器を用いた高速液体クロマトグラフィーによる雨水中の硝酸イオン,亜硝酸イオンの同時定量

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A method for the simultaneous determination of nitrate and nitrite was studied by HPLC with an electrochemical detector (BCD). Nitrate and nitrite were separated by TSK gel QAE-2SW (150mm×4mm i. d.) and nitrate was reduced to nitrite on a copper-cadmium column (100mm×5mm i. d.). Nitrite was detected by ECD with a glassy carbon electrode as a working electrode, the potential of which was set at +0.9V vs. Ag/AgCl. The mobile phase consisting of 0.1 M phosphate buffer containing 0.05 M sodium chloride (pH 7.0) was pumped at a flow rate of 0.5 ml/min. Injection volume was 20 μl. According to this method, the retention times of nitrate and nitrite were 8.0 and 9.8 min, respectively. Calibration curves for both anions were linear in the range of 0.2〜40 ng as N and relative standard deviation (n=5) were less than 2%. We applied this method to the determination of nitrate and nitrite in rainwater.
社団法人日本分析化学会の論文
1986-11-05