バニリン硫酸法による第三級アルコールの吸光光度定量

概要

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It was found out that a tertiary alcohol such as t-butanol, 3-pentanol, and 3-methyl-3-pentanol gave a red color by treatment with a vanillin and high concentration of sulfuric acid in a boiling water bath. Analytical procedure is as follows; To 2.0ml of the test solution containing (5〜50) *g of tertiary alcohol, add 1.0ml of 0.7w/v% vanillin solution (which is dissolved 0.7 g completely in 20 ml of ethanol, and diluted to 100ml with water), and 5ml of 80% sulfuric acid. Heat the mixture in a boiling water bath for 20 min. After cooling to room temperature, measure the absorbance against the reagent blank. Absorption maxima for f-butyl alcohol, 3-pentanol, and 3-methyl-3-pentanol are 505 nm, 515 nm, 520 nm, with the molar extinction coefficient of 9.94, 13.4, 13.8 × 10^3 mol^<-1> cm^<-1> dm^3, respectively.
1982-10-05