細管式等速電気泳動法におけるカウンター・フローの効果

概要

論文の詳細を見る
A syring-type conterflow device for isotachophoresis has been developed to separate ions that differ only little in mobility. The device was installed in a Shimadzu Isotachophoretic Analyzer IP-1, in order to study the effects of the conterflow on the separation efficiency. The migration current was set to 100μA; the capillary tube was 50cm long; the sample was amixture of aqueous solutions of 0.01M sodium oxalate, 0.01M sodium formate and 0.01M sodium citrate. The rate of counterflow was varied in 6 steps of 6, 12, 50, 100 and 200μl/h, by changing the plunger speed. Thus, separation efficiency has been studied as a function of sample volume and conterflow rate. A mixed zone of formate and citrate was observed for asample volume above 8μl when counterflow was not applied. This mixed zone was eliminated when counterflow was applied at 200μl/h for 15 min. The effective length of the capillary tube can be increased by 40% by applying a counterflow.
社団法人日本分析化学会の論文
1975-11-10