カーボン電極上でのペルオキシダーゼの電子移動反応(<特集>表面・界面・薄膜と分析化学)

概要

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Electrode reactions of horseradish peroxidase (HRP) at pyrolytic graphite (PG) electrodes were examined by cyclic voltammetry. HRP gave a redox wave around -0.3 V (vs. Ag/AgCl) at pH 8,which was due to heme of the HRP adsorbed on the electrode : HRP(Fe^<3+>)_<ad> +e→HRP(Fe^<2+>)_<ad>. The addition of H_2O_2 to the HRP solution induced a catalytic reduction current at thc electrode around -0.4 V at pH 8 ; larger catalytic currents were obtained in acidic solutions. This catalytic reduction of H_2O_2 can be given as 2HRP(Fe^<2+>)_<ad>+H_2O2+2H^+→2HRP(Fe^<3+>)_<ad>+2H_2O, where the catalytic reduction took place favorably through the reduction of heme in HRP. but not through the free heme moiety dissociated from HRP.
社団法人日本分析化学会の論文
1991-11-05