ビタミンB_<12>の吸着ストリッピングボルタンメトリー

概要

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Vitamin B_<12> yields a polarographic catalytic wave at ca. -1.6 V vs.SCE. Vitamin B_<12> in river-water samples was determined by adsorptive stripping voltammetry in order to use this catalytic wave. The supporting electrolyte used was 0.01 M Na_2HPO_4+0.01 M NaH_2PO_4. A preconcentration potential of -1.2 V was applied for 5 min. A differential pulse polarograph was used to monitor the catalytic currents. The calibration curve was linear up to 14 nM, and the detection limit was 0.7 nM. The relative standard deviation was 7.8% with 5 nM vitamin B_<12>. A concentration of 1 μM Cu (II), Cd (II), Zn (II), Ni (II), Co (II) or sodium dodecyl sulfate did not interfere with this method. Although the dissolved vitamin B_<12> (filtered with 0.45 μm filter paper) in river water could not be detected, the total amount of vitamin B_<12> colud be determined. Vitamin B_<12> in Shimanto River, Matsuba Riber and Niida River was 2.3,2.8 and 1.2 nM, respectively.
社団法人日本分析化学会の論文
1994-04-05