アノイリナーゼIに関する研究 : (III)精製アノイリナーゼIの性状

概要

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As shown in the previous reports, a highly purified preparation of thiaminase I of Bacillus thiaminolyticus was obtained. In this paper some properties of the purified thiaminase preparation are reported. The thiamine-decomposing reaction by the purified thiaminase I showed first order kinetics. The optimal temperature was 50℃, and the optimal pH was 6.0 when pyridine was added to the reaction system. The enzymic activity was increased by Mn^<..>, Mg^<..> or Ca^<..>, etc., and inhibited by Cu^<..>, Ni^<..> or Zn^<..>, etc. It was activated by some chelating reagents, such as citrate, urea or histidine, and inhibited by some others, such as EDTA or NaCN. Furthermore, the addition of such an inorganic substance as sodium sulfide or sodium thiosulfate highly increased the enzymic activity and also its stability.
日本ビタミン学会の論文