アノイリナーゼIに関する研究 : (I)Bacillus thiaminolyticusの産生するアノイリナーゼIの精製法の予備実験

概要

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Purification of thiaminase I of B.thiaminolyticus was undertaken by the following procedures. At first, a method of purification of toxin was applied to the treatment for the bouillon culture of the bacteria. Secondly, the fraction precipitated at 0.3-0.6 ammonium sulfate saturation was separated as a crude enzyme. Furthermore, since it was observed that thiaminase I was strongly adsorbed on anion exchangers, Amberlite IRA-400 column was used, and ten-fold concentrated fraction, compared with the crude enzyme, was obtained. Precipitation did not occur in a solution of the purified thiaminase I by the addition of trichloroacetic acid or sulfosalicylic acid solution, and biuret reaction was positive.
日本ビタミン学会の論文