アノイリナーゼIに関する研究 : (II)アノイリナーゼIの精製

概要

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B.thiaminolyticus was cultivated for 4 days in the Sakakibara-Yokota's synthetic medium and the purification of thiaminase was undertaken, using the culture filtrate. After the filtrate was stirred in Warning blender and concentrated through the osmotic concentration procedure of Sakakibara and Katsumata, a crude enzyme was precipitated at 0.3-0.6 ammonium sulfate saturation. The crude enzyme was treated with Amberlite IRA-400 column and the enzyme adsorbed on the resin was eluted with 1 M ammonium sulfate solution. The purified enzyme obtained showed a typical simple protein picture on the ultraviolet-spectra and had a high thiamine decomposing ability. The purified enzyme was positive in biuret and Folin's reactions and was precipitated by sublimate solution.
日本ビタミン学会の論文