細菌のビタミンB_1合成および分解に関する研究 : (V)2-ALKYLTHIOTHIAMINEとそのピリミジン部類似体の細菌の発育とビタミンB_1合成に与える影響

概要

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2-Methylthiothiamine was an inhibitory antagonist against a thiamine-requiring mutant of Escherichia coli strain 70-23 and the molar ratio of inhibition (inhibitor/thiamine) was of the order of 100-1000. The corresponding 2-ethylthiothiamine showed much less inhibitory potency than the methyl derivative on the same molar ratio. On the other hand, a thiamine-pyrimidine-requiring mutant strain 70-17 could use the methyl derivative alone for growth in place of pyrimidine at the concentrations over 10^<-6> M/l. The mutant was, however, inhibited by the compound in the presence of less than 10^<-9> M/l of thiamine. Pyrimidine moiety of this compound had not the inhibitory activity for both strains and was utilized for synthesis of the compound by their parent strain ATCC 9637. It was, therefore, probable that the compound was competitive for thiamine at the stage of phosphorylation of thiamine or more posterior stage.
日本ビタミン学会の論文