組織によるビタミンAのエステル化の研究 : とくに肝臓および膵臓のビタミンAエステル化能測定の条件の検討

概要

論文の詳細を見る
On the determination of synthesizing activity of vitamin A ester, the several conditions were studied. The activity was recognized in the liver and pancreas homogenate of the rat, which were incubated at 38℃ for 30 or 60 mininutes with the substrate mixture containing vitamin A alcohol and palmitic acid in ethanol. The reactions were carried out adequately in the enzyme concentration containing 10 mg of pancreas tissue or 100 mg of liver tissue for 30μg of vitamin A alcohol in the reaction mixture. The ethanol in substrate solution have shown inhibitory effect at 0.5% of the concetrations for liver tissue and at 8.0% for pancreas tissue, The esterase reaction in the both tissues on vitamin A was found at pH 7.0 to be available for esterification, while at pH 8.5 for hydrolysis.
日本ビタミン学会の論文
1964-06-25