天然資源よりビタミンB_<12>結晶の製造に関する研究 : (XII)アルコール蒸溜廃液のメタン醗酵処理物を原料とするビタミンB_<12>結晶の製造案

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Based on the fundamental studies on the purification of vitamin B_<12> already reported, two plans for the preparation of crystalline vitamin B_<12> from methane fermentation products of distillers' wastes were worked out as follows : (1) The bacterial cell fraction obtained from the supernatant liquor in the methane fermentation tank is extracted with boiling water containing KCN, followed by filtration. Vitamin B_<12> in the filtrate is adsorbed on sulfonated coal. An excess of NaCN is added to the eluate of vitamin B_<12> from the coal to convert the vitamin to its dicyanide. Then the dicyanide is adsorbed on the strong basic porous anion exchange resin, followed by elution of vitamin B_<12> with a dilute acid. The eluate is passed through another column of the resin to remove impurities. Vitamin B_<12> in the effluent is extracted with a lower fatty acid and is transferred into aqueous layer by addition of butyl acetate and water. Thus, vitamin B_<12> might be crystallized from aqueous acetone. (2) An alternative plan consists of extraction of vitamin B_<12> with an organic solvent from the activated sludge of the supernatant liquor described above, adsorption on the strong acidic porous cation exchange resin, precipitation with a metallic compound, solvent extraction with a lower fatty acid, followed by transferring the vitamin into aqueous layer by addition of CaCO_3 and water, and a final crystallization from aqueous acetone.
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天然資源よりビタミンB_<12>結晶の製造に関する研究 : (XII)アルコール蒸溜廃液のメタン醗酵処理物を原料とするビタミンB_<12>結晶の製造案

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