天然資源よりビタミンB_<12>結晶の製造に関する研究 : (IX)アニオン交換樹脂によるDICYANOCOBALAMINの吸着

概要

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The author has found that dicyanocobalamin, a derivative of vitamin B_<12>, is adsorbed on the anion exchange resin, which has no affinity to vitamin B_<12>. From this finding, the following very simple, yet quick and effective, method of purifying vitamin B_<12> has been invented. An aqueous solution of vitamin B_<12> from natural resources is passed over a column of the resin, and a part of the impurities is adsorbed. An excess of NaCN is then added to the vitamin B_<12> containing effluent to convert the vitamin to dicyanocobalamin. The resulting solution is passed over another column of the same resin. During this second passage through the column, the dicyanocobalamin in the solution is selectively adsorbed, and thus freed from the other impurities, which remained unadsorbed after the two passages. The original vitamin B_<12>, which is adsorbed on the resin in the form of dicyanocobalamin, is recovered from the resin by eluting with a dilute acid solution containing an organic solvent. By carrying out the procedure described above, the vitamin B_<12> in the original starting solution is readily isolated and freed from almost all impurities contained therein.
日本ビタミン学会の論文