ビタミンAおよびDの濾紙クロマトグラフによる微量定量について

概要

論文の詳細を見る
The microdetermination of fat-soluble vitamins by paper chromatography, which was introduced by Datta and developed by Sahashi and Suzuki, was retested using calcium-adsorbed filter paper. The optimal amounts of vitamin A alcohol and ester were found to be 0.03-0.25μg. Each fraction obtained by paper chromatography could be recovered up to 80-90% by extraction. In the case of blood plasma it was found necessary for separating both components preliminarily to treat it with cold KOH-ethanol, whereby the natural ester of vitamin A remained intact. But each component, which had been added to plasma, was recovered about 75-85%. For extracting both components of vitamin A in liver an appropriate procedure was described, but the recoveries of the added components were also about 80-90%. The Rf values of both components of vitamin A being markedly different according to the coexisting substances, the spot of each fraction must be decided by addition test. Both vitamin D_2 and D_3 behaved completely similarly in paper chromatography. The optimal amount of the vitamin was 8-36μg and the recovery of the added vitamin about 80%. From the mixture of vitamin A and D, each vitamin could not be separated. The optimal amount of α-tocopherol was found to be 11-45 μg.
日本ビタミン学会の論文