麦芽根中の核酸分解酵素系とその応用に関する研究 : (第2報)リボ核酸分解酵素系について(その2)

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The optimum pH of RNase extracted with water or a natural salt solution was around 5. This optimum pH shifted to 6 with a heat treatment and the activity presentes at around pH 5 mostly disappeared at pH 6. From this fact two tipes of RNase were considered to exist in barley rootlets. The optimum pH of RNase (RNase II) extracted at pH 2〜3 was 4.5 and RNase II was almost inactive at 6.0. RNase II degraded RNA to nucleoside 2', 3'-cyclic phosphates of all four bases, which were then slowly hydrolysed to nucleoside 3'-or 2'-monophosphates. RNase II was more labile to heat than RNase I, which degraded RNA to nucleoside 5'- monophosphates.Phosphomonoesterase and phosphodiesterase were also found in barley ectract.The presence of nucleosidase and nucleoside deaminase in the barley extract was studied by paper electrophoresis.
公益社団法人日本生物工学会の論文
1968-08-25