ペーパークロマトグラフイによるタンニン分解酵素生産条件の解析(その1) : 黴類のタンニン分解酵素に関する研究(第5報)

概要

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The enzyme solution extracted from tannin(T)- or gallic acid(G)-containing "Fusumakoji" and mycelium of Penicillium sp. No. 80 B', which had grown on the liquid medium by replacement-stationary culture, was used for this experiment. The paper chromatographic analaysis on enzyme formation was carried out by the use of paper chromatogram, which showed the process of enzymatic tannin decomposition.1) Tannin-decomposing enzyme(TDE) was found to be an inducible (or adaptive) enzyme, for the mold never produced the enzyme unless T or G wasad ded to the medium. Pyrogallol had no or little effect on the enzyme formation.2) The production of TDE by the mold decreased as the disappearance of T or G in the medium. After the complete consumption of T or G, the activity was no more detected.3) Some properties of a substance which may be assumed to be an intermediate product of tannin hydrolysis were dicussed.4) The enzyme formation by the mold was discussed in this paper, and its was suggested that G is an essential part of the inducer.
社団法人日本生物工学会の論文
1959-03-15