液体培養におけるタンニン分解酵素の生産条件について(その2) : 黴類のタンニン分解酵素に関する研究(第10報)

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Some conditions of the tannase formation by Penicillium sp. No. 80B' were examined in shaken cultures with CZAPECK-DOX solution contatining 0.5% tannin as the secondary medium.The results obtained were as follows.1) Age of pre-culture had an effect on the adaptability of the mold to tannin ; tannase formation by old cells was faster than that by young cells.2) For abundant formation of tannase, it was necessary to use nitrogen sources such as NaNo_3 and (NH_4)_2SO_4 which were found to be more effective than organic ones such as aspartic and glutamic acids.3) Addition of 2,4-dinitrophenol to the secondary culture did not cause considerable inhibition of enzymatic hydrolysis of tannin to gallic acid, but inhibited the subsequent decomposition of gallic acid. In this case, tannase formation was poor, but its activity lasted comparatively long.4) Iron, in the form of FeSO_4・7H_2O, had little effect on the tannase formation.5) Stimulating effect of various kinds of phenols upon tannase formation was examined, and it was presumed that gallic acid is the smallest structural unit effective as inducer.6) Various tannins such as Turkish allotannin, sumach tannin and myrobalan tannin, which are similar to Chinese gallotannin in structure, could give rise to tannase formation.The above results are consistent with the view that this enzyme is an inducible (or adaptive) enzyme. In designation of this enzyme, the author has an intention to propose that "gallo-" is prefixed to tannase to compose the name gallo-tannase to this enzyme.
社団法人日本生物工学会の論文
1961-03-15

液体培養におけるタンニン分解酵素の生産条件について(その2) : 黴類のタンニン分解酵素に関する研究(第10報)

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