Corynebacterium glutamicum変異株の性質と安息香酸よりのカテコール蓄積機構 : 細菌による安息香酸よりのカテコールの生産(第2報)

概要

論文の詳細を見る
Resting cells of bM 9 accumulated catechol in a reaction mixure containing benzoic acid. Optimal pH for the accumulation was 7.0 or 8.0.Catechol degrading activity of bM 9 cells was considerably lower than that of parent cells. Oxygen uptake of bM 9 cells in the presence of benzoic acid or catechol was smaller than that of parent cells. Low activity of degradation against catechol (or benzoic acid) of bM 9 was thought to cause the accumulation of catechol.Degradation of benzoic acid by growing parent cells was inhibited by glucose in the medium, whereas the degrading activity of bM 9 was insensitive to glucose. Induction of the benzoic acid degrading activity of resting cells was affected by the media components. The activity of parent cells grown on nutrient broth supplemented with glucose was lower than those grown without added glucose. Induction of the activity of bM 9 was not affected by the addition of glucose. p-Hydroxybenzoic acid was apparently effective for the induction of benzoic acid degrading activity for both bM 9 and parent when it was added into the media containing yeast extract.
社団法人日本生物工学会の論文
1976-11-25