植物種子発芽体の核酸分解酵素に関する研究 : (第5報)小麦, アワ発芽体および麦芽根核酸分解酵素の精製とその性質

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概要

• 論文の詳細を見る

• Crude enzymes extracted from wheat, barley and millet sprouts were purified by several procedures and separated into fractions of five nucleases (PD_1-PD_5), two phosphatases (PM_1-PM_2) and one polynucleotide phosphorylase by the column chromatography on DEAE-cellulose. Then they were purified by approximately 1,000 folds of the nucleases, 500 folds of the phosphatases and 400 folds of the PNPase with absorbancy at 280 mμ.1) Three out of the five nucleases (PD_1,PD_2 PD_4) and the two phosphatases (PM_1,PM_2) were the same as those of the three nucleases and two phosphatases separated from mung bean sprouts; PD_1 was the endonuclease with the optimum pH at 4.5. PD_2 and PD_4 were exonuclease with the optimun pH values at 5.0 and 8.0. In PM_1 and PM_2 the optimum pH was 5.0 and 7.5 respectively.2) The optimum pH of PD_3 and PD_5 were 5.5 and 8.0,and the temperature optimum was 60℃ for both. They were considerably heat labile (60% of their activities vanished in 30 min at 60℃). They are stable at pH values from 4.5 to 7.0. Their activities were inhibited by metal ions and activated by a few chelating agents.3) PD_3 and PD_5 showed the activities on RNA, DNA and bis (p-nitrophenyl) phosphate, but on RNAcore and thymidine-5'p-nitrophenyl phosphate they did not show any activities. In their hydrolysates of RNA, no 5'-mononucleotides were detected and more 3'-pyrimidine mononucleotides were found than 3'-purine mononucleotides. From these results, PD_3 and PD_5 may be classified as endonuclease specific to bases and substrates.4) The optimum pH and temperature of PNPase were 5.5 and 50℃. It is considerably heat stable (only 20% of the activity vanished in 30 min at 60℃) and it was also stable at pH 5.0-7.0. It was activated remarkably by Mg^<++>, Ca^<++>, Co^<++>, Mn^<++> and PO^<3->_4. It strongyl reacted on 5'-ADP but weakly on 5'-CDP.

• 社団法人日本生物工学会の論文
• 1969-02-25

著者

• 川田 寛

  日本化学飼料株式会社

• 片谷 健一

  日本化学飼料株式会社

• 秋本 政明

  日本化学飼料株式会社

• 西本 健市

  日本化学飼料株式会社

関連論文

• 植物種子発芽体の核酸分解酵素に関する研究 : (第5報)小麦, アワ発芽体および麦芽根核酸分解酵素の精製とその性質
• 植物種子発芽体の核酸分解酵素に関する研究 : (第4報)小麦, アワ発芽体および麦芽根抽出液の酵素的性質
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