植物種子発芽体の核酸分解酵素に関する研究 : (第1報)核酸分解酵素の検索

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The activities of nuclease and phosphatase in sprouts of 25 species of plant seeds were studied.1) In all of the sprouts, the nuclease and phosphatase activities were found. Most of these nucleases could hydrolyze RNA into 5'-mononucleotides characterized by periodic Schiff's reaction on paper-electrophoresis.2) The nuclease activity is observed in seeds themselves. However, the activity was increased remarkably by germination, and it was at its maximum in three or four day after germination. Similar tendencies were observed in the phosphatase activity and the content of RNA.3) The optimum pH of nucleas and phosphatase in the crude enzyme solution extracted from sprouts was found in both acidic (pH 4.5) and alkaline (pH 7.5) regions.4) The distribution of the activities of ribonuclease, phosphodiesterase and phosphomonoesterase among the sections or a sprout was in the following order : root>shoot>cotyledon (or endosperm).
社団法人日本生物工学会の論文
1969-01-25