亜硫酸パルプ廃液における酵母の混合培養について(生物化学工学特集号-I廃液処理)

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Using seven strains of yeasts, i. e., Pichia sake form. α and Pichia farinosa (selected by the author in the preceding paper), Candida pelliculosa (isolated by the author), Mycotorula sp., Candida utilis and Rasee XII as test organisms, pure culture and mixed culture agitation tests in sulfite waste liquor media containing (NH_4)_2SO_4 and KH_2PO_4 were carried out. The yeast yields and the rate of sugar consumption of these yeasts in both cultures were investigated comparativelhy. The experimental results are shown in Table 1〜3. The rate of sugar consumption by the culture of Candida utilis, which has lower ability to consume sugar in sulfite waste liquor medium than the other yeast strains employed in these experiments excepting Rasse XII in pure culture, in cumbination with one strain each of the following yeasts, i.e., Pichia farinosa, Pichia sake form. α, Hansemula anomala, Mycotorula sp. and Candida pelliculosa, which have higher ability to consume sugar in the sulfite waste liquor medium than Candida utilis in pure culture, exceded the rate attained by the pure culture of Candida utilis. The yield of yeast cells in a mixed culture of Candida utilis and Mycotorula sp. exceded the yield attained by either of the strains in pure culture. These results obtained from the mixed cultures are interesting from the standpoint of the biological treatment of sulfite waste liquor and the increasing of yeast cell yield from the liquor.Candida utilis and Mycotorula sp. were cultured seperately with agitatio for 45 horus at 30℃ in the sulfite waste liquor media containing about 2% sugar. Adding their culture filtrates to the sulfite waste liquor media, the effects of both the culture filtrates on yeast growth were tested. The addition of the culture filtrate of Candida utilis was effective for the growth of Mycotorula sp., but addition of the culture filtrate of Mycotrorula sp. had little effect on the growth of Candida utilis. Using the culture appratus with semipermiable membrance developed by Mc Veigh and Brown, the mixed culture of two strains of yeasts, Candida utilis and Mycotorula sp., in the sulfite waste liquor media were carried out without agitation. As a result, it was found that the growth of Mycotorula sp. was promoted by culturing it in combination with Candida utilis, while the growth of Candida utilis was little promoted by culturing it in combination with Mycotorula sp.
1965-06-25

著者

赤木盛郎
三重大学農学部

亜硫酸パルプ廃液における酵母の混合培養について(生物化学工学特集号-I廃液処理)

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