チアミン生合成過程における炭酸固定反応の有無について

概要

論文の詳細を見る
It has been proposed that the pyrimidine moiety of thiamin and purine share the early part of their biosynthetic pathways in bacteria. Newell and Tucker had reported that the last common intermediate is 5-aminoimidazole ribonucleotide (AIR) on the basis of its satisfaction on the requirements both for a purine and for pyrimidine moiety of thiamin of some double-requirement mutants of Salmonella typhimurium. We investigated the possibility of 5-aminoimidazole-4-carboxylic acid ribonucleotide (AICAR) as common inter-mediate by using radioactive bicarbonate. Radioactive carbon of bicarbonate was not incorporated into pyrimidine and thiazole moieties of thiamin in Escherichia coli and Saccharomyces cerevisiae, while the carbon was incorporated into AMP from RNA. These results show that their biosynthetic routes do not contain CO_2-fixation reaction and AICAR is not a last common inter-mediate in E. coli.
日本ビタミン学会の論文
1981-11-25