高速液体クロマトグラフィーと微生物学的定量法とを組み合わせた方法によるヒト血清中コバラミン同族体の分画定量

概要

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Cobalamins in human serum were separated using HPLC gradient system after extraction with N- ethylmaleimide and citrate-phosphate buffer system. Each cobalamin analogue in the elute was con verted to cyanocobalamin (CN-Cbl) by the addition of KCN, and the cobalamin contents were deter mined by a microbiological assay with Lactobacillus delbrueckii subsp. lactis ATCC 7830. This method was applied to determination of serum cobalamin analogues in healthy volunteers. The ratios of each cobalamin analogue in the serum of the male volunteers were methylcobalamin (CH3-Cbl): 66.5 ± 12.3%, hydroxocobalamin (OH-Cbl): 17.1 ± 9.4% and adenosylcobalamin (Ado Cbl) : 14.1 ± 5.8%. The ratios for female volunteers were CH_3-Cbl: 72.4 ± 9.3%, OH-Cbl: 12.3 ± 8.6% and AdoCbl: 9.8 ± 5.8%.
日本ビタミン学会の論文
2000-03-25