セミミクロカラムを用いた高速液体クロマトグラフィー : カラムスイッチングシステムによるヒト血清および血清限外ろ過液中のワルファリンの定量

概要

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A simple and sensitive reversed-phase high-performance liquid chromatographic method was developed for the analysis of warfarin (WF) in both human serum and serum ultra filtrate. WF and intemal standard (I.S.) were extracted from the serum by simple deproteinization and filtration with a membrane filter. The Column-switching system utilized the short polymer-coated mixed-function(PCMF) phase deproteinization column coupled with a C_<18> semi-micro analytical column using a mobile phase of acetonitrile, water and NH_4H_2P0_4, followed by a post-column photochemical reaction and fluorimetric detection. The extraction recovery was greater than 80% and the WF and I. S . were separated from the endogenous components in the serum and serum ultra filtrate. The calibration curve was linear from 5 to 1000 ng/mL of serum and I to 150 ng/ mL of the serum ultra filtrate. The limit of quantification was 5 and I ng/mL for found to be the serum and serum ultra filtrate, respectively. The inter and intra-day coefficients of variation for the serum and serum ultra filtrate ranged less than 10%, respectively. WF was found to be the stable in the serum for a 4-month period during storage at -20℃.
日本医療薬学会の論文
1999-04-10