細菌の突然変異修復遺伝子発現システム(umuシステム)を用いた極低周波磁界によるDNA損傷の評価

概要

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The effect of a extremely low frequency magnetic field (ELFMF) on living biological cells was investigated by using a highly sensitive new mutagenesis method. A bacterial gene expression system for mutation repairing (umu system) was employed for the sensitive evaluation. Salmonella typhimurium TA1535 (pSK1OO2), a mutagensensitive bacterium that possesses a gene expression system with an SOS response to mutagenesis (umu operon), fused with the β-galactosidase gene, was exposed to an ELFMF in a specially designed magnetic field loading chamber. The experimental results showed that the umu assay could be effectively applied to the evaluation of damage in DNA molecules by exposure to an ELFMF. However, no significant differences were observed in the umu gene expression intensity under experimental condition.
社団法人日本磁気学会の論文
1999-10-01