高速液体クロマトグラフィーによる清酒中の核酸関連物質の分析

概要

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A negative correlation was observed between ultraviolet absorbance and quality of sake which was appraised by a sensory test. High pressure liquid chromatography (HPLC) was applied to investigate the ultraviolet absorbing constituents and three peaks were correlated with the quality of sake. The ultraviolet absorbing constituents were extracted from sake by treating with activated carbon, and then components of the three peaks were fractionated by gel chromatography with Sephadex G-15. The peaks were identified as uridine, hypoxanthine, and tyrosine by R_f values on thinlayer chromatography, HPLC retention times, UV and IR absorption spectra.On the other hand, high pressure liquid chromatographic analysis of nucleic acid components was studied. Nucleotide monophosphates, nucleosides, and their bases were separated simultaneously in less than 90min, on a column packed with polyethylene porous polymer gel (Shodex OHpak B-804) and eluted with a linear gradient of citric acid and ammonium phosphate buffer with varying pH, from 2.7 to 3.6.
公益社団法人日本生物工学会の論文
1980-07-25