限外ろ過法による菌体の洗浄

概要

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The harvesting and washing of cells by ultrafiltration (UF) were studied with E. coli, K-12 broth in nutrient medium and TS-3000 membrane with a nominal cut -off molecular weight of 3×10^6 set in a thin-channel type UF cell. In the absence of high molecular weight components other than cells the broth could readily be concentrated, but when bovine serum albumin (BSA) was added to the broth, the permeability of BSA through TS-3000 varied with the circulation rate of the feed solution and the initial pure water flux. By changing these two parameters, the optimal operating conditions for passing BSA through TS-3000 membrane were investigated.
公益社団法人日本生物工学会の論文
1984-07-25