稻熱病斑の組織化學的研究 II : ヂアゾ試藥により赤變する葉の細胞膜中の物質について

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In the previous paper, it was suggested that the browning phenomena of lesions might take part in the resistance of leaf tissues of rice to the blast disease, caused by Piricularia oryzae. This paper deals with the constituent of rice plant which may probably give rise to the brown coloration. Results of analysis of amino scids showed that tryptophane and tyrosine could not be considered as the main causes of the brown coloration, despite granules turning red with Ehrlich's aldehyde reagent were usually observed in affected cells of assimilating tissue, suggesting probably the presence of some indole derivatives. The cell membranes of vascular bundle, collenchym, and epidermis turn red to orange with diazo reagent. By means of staining methods using Liebermann's nitroso reaction or Hopfner's reaction, phloroglucinol-HCl, benzidine-HCl, Payen's reaction, ammonium solution, FeCl_3 solution, and conc. H_2SO_4 revealed that these membranes reacted quite similar to chlorogenic acid. In addition chlorogenic acid was detected in the crude extract of leave by means of paper chromatography. Chlorogenic acid appears to exist usually in a form not free but combined with a certain constituent of cell membrane. The presence of chlorogenic acid in the cell membrane may account for the browing of cell membranes of the affected tissues which usually precedes the browning of cell content. Moreover, results of further observations suggest that there exists a possibility that chlorogenic acid is produced and accumulates in affected cells and causes the browning of cell contents.
日本植物病理学会の論文
1953-07-31

稻熱病斑の組織化學的研究 II : ヂアゾ試藥により赤變する葉の細胞膜中の物質について

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