イネごま葉枯病に関する病理解剖学的研究 : II. 子葉鞘に侵入した菌糸の微細構造

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The present paper describes the fine structure of hyphal ce11s of Helminthosporium oryzae which invaded the coleopti1e of two rice varieties, Kameji and Shiga Asahi 27, resistant and susceptible to the brown leaf spot respectively. Leaf tissues were fixed in KMn0 4 (2% in distilled water) and postfixed in a l% solution of phosphate buffered OsO 4. 1. In the susceptible varietyt hehyphae of the fungus remarkably increased its diameter after penetrating the cuticle of epidermal ce11 wa11s, and deve1oped through the middle layer of radial wa11s of epidermal ce11s and then, in most cases, intruded into epidermal ce11s. 2. The ce11 wa11 of young immature hyphae which invaded the parenchymatous ce11s of the resistant and susceptible varieties consisted of only a mucilaginous inner layer, sim11ar to that of immature germ tubes. However, two-1ayered ce11 wa11s were observed in the matured hyphae, electron-dense outer layer (about 0.12μ in thickness) and electron-transparent inner layer (about 0.20μ). Vacuoles in old hyphae deve1oped extensively, some reaching 3μ in diameter, and occupied a greater part of the cytoplasm. 3. In the susceptible variety accumulation of electron-dense substances, 0.5μ thickness was recognized around the hyphae in the radial wa11 of epidermal ce11s. This might be a response product of the host ce11 to the exudate of the fungus. 4.In the parenchymatous tissues of the susceptible variety the hyphal tips swe11ed again after penetrating the ce11 wa11 by a slender hypha, about lμ in diameter. In these newly formed hyphal ce11s many mitochondria and 1ipid bodies were recognized 5. In an affected epidermal ce11 of coleoptiles of the resistant variety, substances which appear to be due to the degeneration of cytoplasm of the host ce11s were recognized around the hyphae. Less amount of these substances was detectable around the hyphae in the susceptible variety. 6. The hyphae of the causal fungus in coleoptile of the resistant variety were genera11y slender, about lμ in diameter. They had large vacuoles in cytoplasm, and finally they showed a vacuous appearance retaining only the outer layer of ce11 wa11s. 7. In the resistant variety, Kameji, chloroplasts in parenchymatous ce11s at first we11ed after the invasion of the causal fungus. Thereafter, the intergrana-1ame11ae exanded and the grana-1ame11ae changed into many sma11 vesicles, 0.1-0.5μ in diameter. Thus the ch1oroplasts were fina11y degenerated. This degenerative process is referred to so-ca11ed vacuo1ar degeneration.
日本植物病理学会の論文
1968-09-30

イネごま葉枯病に関する病理解剖学的研究 : II. 子葉鞘に侵入した菌糸の微細構造

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