稻熱病菌の菌絲發育に適する合成培養液の研究

概要

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The mycelial development of Piricularia Orysae CAV. is generally poor in ordinary synthetic nutrient solutions. The authors carried out cultural experiments of this fungus with several syntetic nutrient solutions in an attempt to set up a formula of somewhat more suitable one for the culture of the present fungus. Based upon the results obtained in the cultural experiments the authors contrived tire following formula of a synthetic nutrient solution : Potassium nitrate (KNO_3) 2.0g Monopotassium phosphate (KH_2PO_4) 0.5g Dipotassium phosphate (K_2HPO_4) 0.5g Magnesium sulphate (MgSO_4・7H_2O) 0.5g Calcium chloride (CaCl_2) 0.1g Ferric chloride (FeCl_3) trace Sucrose 30.0g Redistilled water 1000.0cc One drop of 5 % FeCl_3 solution for 30 cc of the nutrient solution. Hydrogen ion concentration of the present solution was about pH 6.3, and a slight precipitation unavoidably took place. The fungus grew tolerably well in this nutrient solution, and a grayish olive colored mycelial colony developed in 30 cc of this solution by a culture in a thermostat at 28℃ for 30 days was measured to be more than 4 mg in dry weight. The present formula would be recommended as a suitable synthetic nutrient solution for the mycelial development of Piricularia Oysae CAV.
日本植物病理学会の論文
1940-12-20