Quantification of α-Methylene-γ-butyrolactone Extracted from Different Parts of Alstroemeria wilhelmina and Evaluation of it's Antigenicity Using the Guinea-Pig Maximization Test
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概要
- 論文の詳細を見る
To detect the type of contact dermatitis caused due to the handling of Alstroemeria wilhelmina, 1% α-methylene-γ-butyrolactone (α-MBL) dissolved in physiological saline and a five-fold diluted saline solution of original extracts of flowers, leaves and stems of the flower were applied to guinea-pigs for induction treatment, and 0.1% α-MBL physiological saline and a ten-fold diluted solution of the original extracts were applied to the animals as the challenge treatment in compliance with the guinea-pig maximization test (GMT). As a consequence, not only primary irritant dermatitis was observed, but also delayed type allergic contact dermatitis due to Alstroemeria wilhelmina was observed. α-MBL determined in the extracts using high-performance liquid chromatography (HPLC) was found to be the biochemical material cause of the contact dermatitis. The flower region contained α-MBL in the highest concentrations compared with those of the leaves and stems. Therefore, the quantification of α-MBL in the extracts was concluded as being a useful evaluating method for contact dermatitis due to the handling of Alstroemeria.
- 日本衛生学会の論文
著者
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UEDA Atsushi
Department of Physical Science, Hiroshima University
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Wei Chang
Department Of Preventive And Environmental Medicine Graduate School Of Medical And Pharmaceutical Sc
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Ueda Atsushi
Department Of Community Health Nursing School Of Health Sciences Kumamoto University
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ARIMATSU Yoshiki
Ginkyo College of Medical Science
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HARADA Koichi
Department of Hygiene, Kumamoto University School of Medicine
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OHMORI Shoko
Department of Hygiene, Kumamoto University School of Medicine
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WEI Chang
Kumamoto Total Institute of Fitness
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Ohmori Shoko
Department Of Preventive And Environmental Medicine Graduate School Of Medical And Pharmaceutical Sc
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Ohmori Shoko
Department Of Hygiene Kumamoto University Medical School
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Harada Koichi
Department Of Microbiology And Environmental Chemistry School Of Health Sciences Kumamoto University
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Arimatsu Y
Ginkyo College Of Medical Science
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Ueda Atsushi
Department Of Cardiology Hiroshima City Hospital
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Ueda Atsushi
Department Of Hygiene Kawasaki Medical School
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Harada Koichi
Department of Biomedical Laboratory Sciences, Faculty of Life Sciences, Kumamoto University
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