Pollen Dispersal in a Hinoki (Chamaecyparis obtusa) Seed Orchard Detected Using a Chloroplast DNA Marker
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概要
- 論文の詳細を見る
Pollen dispersal was estimated in two test plots in a hinohi (Chamaecyparis obtusa) seed orchard using a chloroplast DNA marker, the spacer region between the trnD and trnY genes, and SSCP (single strand conformation polymorphism). In Plot 1. 2,020 seeds from 40 trees within 30 m of the marker tree were analyzed using the PCR-SSCP method. In Plot 2, 1,850 seeds from 37 trees were analyzed in the same manner. The results revealed that the maximum pollen dispersal distance in the two plots exceeded 25 m. Pollen dispersal appeared to be inversely proportional to the distance from the marker tree. The effective pollen dispersal was suggested to be less than about 20 m in a mature hinoki seed orchard. Adjacent trees had an excessive influence when the pollen density was increased by artificial flower stimulation. Therefore, it was suggested that seed production better resembles ideal random mating when carried out as naturally as possible. In conclusion, the SSCP chloroplast DNA marker was a useful tool for amassing basic information on pollen management in seed orchards of coniferous species.
- 一般社団法人日本森林学会の論文
- 2001-02-16
著者
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Shiraishi Susumu
Faculty Of Agriculture Kyushu University
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Maeda H
Faculty Of Agriculture Kyushu University
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Maeda Hajime
Faculty Of Applied Biological Science Hiroshima University
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Seido Kunihiro
Yamanashi Forestry and Forest Products Research Institute
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Shiraishi Susumu
Faculty Of Agriculture Hokkaido University
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Maeda Hajime
Faculty Of Agriculture Kyushu University
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