Identification of Chloroplast DNA Haplotypes of Abies firma and A. homolepis Using a Polymerase Chain Reaction with Species-Specific Primers
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概要
- 論文の詳細を見る
DNA sequences of the chloroplast spacer region between the trnP and rrnW genes (234 bps) were determined for two Japanese fir species, Abies firma Sieb. et Zucc. and A. homolepis Sieb. et Zucc., using four individuals from each species. No intraspecific variation was found in either species, but interspecific sequence polymorphism was detected between the two species. The interspecific variation was three nucleotide changes, from the 148th to 150th nucleotide position. These three nucleotides were TAC in A. firma and GTA in A. homolepis, which were inverted. In order to identify the cpDNA haplotypes between the two species, this inversion polymorphism was utilized to develop a new marker. Species-specific primers were designed so that the 3 ' ends of the primers would anneal to the mutation site, in order for the two haplotypes to be easily identified by a polymerase chain reaction (PCR).
- 一般社団法人日本森林学会の論文
- 1999-11-16
著者
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Shiraishi Susumu
Faculty Of Agriculture Kyushu University
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Isoda Keiya
Faculty Of Agriculture Kyushu University
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Shiraishi Susumu
Faculty Of Agriculture Hokkaido University
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