Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 μg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
- 社団法人日本農芸化学会の論文
- 2003-09-23
著者
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Irie Takao
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Enei Hitoshi
Lwate Biotechnology Research Center
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Saito Kumiko
Lwate Biotechnology Research Center
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Watanabe T
Wood Research Institute Kyoto University
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Honda Yoichi
Wood Research Institute Kyoto University
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WATANABE Takashi
Wood Research Institute, Kyoto University
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IRIE Toshikazu
lwate Biotechnology Research Center
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SATO Toshitsugu
lwate Biotechnology Research Center
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KUWAHARA Masaaki
Institute of Wood Technology, Akita Prefectural University
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Kuwahara M
Institute Of Wood Technology Akita Prefectural University
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Kuwahara Masaaki
Institute Of Wood Technology Akita Prefectural University
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