Reaction of Chemically Modified Lignin Peroxidase of Phanerochaete chrysosporium in Water-miscible Organic Solvents
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概要
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LiP of Phanerochaete chrysosporium was chemically modified with MPSS, and acetic and benzoic acid N-hydroxysuccinimide esters to increase the activity in organic solvents. IEF demonstrated the LiP was chemically modified with these modifers. The modification of LiP was also confirmed by GFC and anion-exchange chromatography analyses. In addition, the modified LiP retained its original activity in aqueous solution. Furthermore, these modified LiPs had higher 3,3'-dimethoxybenzidine oxidation activity than the native LiP in aqueous 70% water-miscible organic solutions including ethylene glycol and diethylene glycol. However, the activities of these modified enzymes were found to be negligibly low in water-immiscible organic solvents such as benzene and toluene. Furthermore, the effects of these introducing groups and the properties of organic solvents on the reaction of LiP in water-miscible organic solvents were investigated. The reaction of LiP modified with aliphatic acid was found to depend on the viscosity of the reaction system containing 70% water-miscible organic solvents. This suggests that the viscosity of the reaction system containing water-miscible organic solvents is important for the reaction of LiP modified with aliphatic acids.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
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KUWAHARA MASAAKI
Wood Research Institute, Kyoto University
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Watanabe T
Wood Research Institute Kyoto University
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Honda Yoichi
Wood Research Institute Kyoto University
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WATANABE Takashi
Wood Research Institute, Kyoto University
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YOSHIDA Shinichi
Wood Research Institute, Kyoto University
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Kuwahara M
Institute Of Wood Technology Akita Prefectural University
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Yoshida Shinichi
Wood Research Institute Kyoto University
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Kuwahara Masaaki
Wood Research Institute Kyoto University
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