Characterization of Partially Truncated Human Midkine Expressed in Pichia pastoris(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Recombinant human midkine (rh-midkine) was expressed under the control of the AOX1 gene promoter in Pichia pastoris. Approximately 640 mg of rh-midkine was secreted into one liter of medium of the high cell-density fermentation. The protein processing of the rh-midkine was done efficiently and correctly in P. pastoris, and O-mannosylation was not detected in the purified rh-midkine. However, only about 30% of the purified rh-midkine was intact. The other ones lost 5-12 amino acid residues from the amino-termini, provably by proteolysis. Even the mixture of these truncated midkines could promote CHO cell proliferation as well as the authentic rh-midkine.
- 社団法人日本農芸化学会の論文
- 2002-06-23
著者
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Tohma-aiba Yumiko
Meiji Cell Technology Center Meiji Milk Products Co. Ltd.
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MURASUGI AKIRA
Meiji Cell Technology Center, Meiji Milk Products Co. Ltd.
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Murasugi Akira
Meiji Cell Technology Center Meiji Milk Products Co. Ltd.
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Murasugi Akira
Meiji Cell Technology Center, Meiji Milk Products Co., Ltd.
関連論文
- Production of Recombinant Human Midkine in Yeast, Pichia pastoris
- Comparison of Three Signals for Secretory Expression of Recombinant Human Midkine in Pichia pastoris(Microbiology & Fermentation Technology)
- Efficient Production of Recombinant Human Pleiotrophin in Yeast, Pichia pastoris(Microbiology & Fermentation Technology)
- Characterization of Partially Truncated Human Midkine Expressed in Pichia pastoris(Microbiology & Fermentation Technology)
- An Approach to the Removal of Yeast Specific O-Linked Oligo-Mannoses from Human Midkine Expressed in Pichia pastoris Using Site-Specific Mutagenesis