Comparison of Three Signals for Secretory Expression of Recombinant Human Midkine in Pichia pastoris(Microbiology & Fermentation Technology)

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概要

• 論文の詳細を見る

• The secretion signals of Saccharomyces cerevisiae α mating factor, human midkine itself, and Pichia pastoris acid phosphatase, were tried for the expression of human midkine under the control of the AOX1 gene promoter in P. pastoris. Approximately 28 mg/l, 1.5 mg/l, and 0.2 mg/l of midkine were secreted by using the α mating factor pre-pro-sequence, the midkine signal sequence, and the phosphatase signal sequence in flask cultures, respectively.

• 社団法人日本農芸化学会の論文
• 2001-10-23

著者

• Tohma-aiba Yumiko

  Meiji Cell Technology Center Meiji Milk Products Co. Ltd.

• MURASUGI AKIRA

  Meiji Cell Technology Center, Meiji Milk Products Co. Ltd.

• Murasugi Akira

  Meiji Cell Technology Center Meiji Milk Products Co. Ltd.

• Murasugi Akira

  Meiji Cell Technology Center Meiji Milk Products Co. Ltd.:(present Address)nutritional Foods Develop

関連論文

• Production of Recombinant Human Midkine in Yeast, Pichia pastoris
• Comparison of Three Signals for Secretory Expression of Recombinant Human Midkine in Pichia pastoris(Microbiology & Fermentation Technology)
• Efficient Production of Recombinant Human Pleiotrophin in Yeast, Pichia pastoris(Microbiology & Fermentation Technology)
• Characterization of Partially Truncated Human Midkine Expressed in Pichia pastoris(Microbiology & Fermentation Technology)
• An Approach to the Removal of Yeast Specific O-Linked Oligo-Mannoses from Human Midkine Expressed in Pichia pastoris Using Site-Specific Mutagenesis

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