Design of a Fluorescent Electrophoretic Mobility Shift Assay Improved for the Quantitative and Multiple Analysis of Protein-DNA Complexes(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
We describe a protocol for the fluorescent electrophoretic mobility shift assay improved for the quantitative analysis of protein-DNA complexes. Fluorescent-labeled oligonucleotide probes incubated with nuclear proteins were followed by electrophoresis. The signals for protein-DNA complexes were measured and normalized with fluorescent-labeled marker using fragment analysis software. This assay proved reliable measurement and multiple detection of DNA binding proteins.
- 社団法人日本農芸化学会の論文
- 2002-12-23
著者
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Endo S
Biological Sciences Department Toray Research Center Inc.
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Endo Sumiyo
Biological Sciences Department Toray Research Center Inc.
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Akiyama Hideo
Biological Sciences Department Toray Research Center Inc.
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Kanai Shozo
Biological Sciences Department Toray Research Center Inc.
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Hirano Masahiko
Department Of Endocrinology And Metabolism National Research Institute For Child Health And Developm
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Hirano Masahiko
Biological Science Department Toray Research Center Incorporation
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ONIZUKA Takuo
Biological Science Laboratories, Toray Research Center, Inc.
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Onizuka Takuo
Biological Sciences Department Toray Research Center Inc.
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Akiyama Hisayoshi
Biological Sciences Department Toray Research Center Inc.
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Hirano Masahiko
Department Of Health Science Gunma University School Of Medicine
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Akiyama Hideo
Biological Science Laboratories, Toray Research Center, Inc.
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Kanai Shozo
Biological Science Laboratories, Toray Research Center, Inc.
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Endo Sumiyo
Biological Science Laboratories, Toray Research Center, Inc.
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