Modulation of Cytochrome P4501-mediated Bioactivation of Benzo[a]pyrene by Volatile Allyl Sulfides in Human Hepatoma Cells(Food & Nutrition Science)

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概要

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• Allyl sulfides such as diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), typical flavor components of Allium vegetables, have been shown to inhibit benzo[a]pyrene (B[a]P)-induced carcinogenesis in animal models. As a possible mechanism of this inhibition, the effect of these volatile substances on cytochrome P450 (CYP)1 (CYP1A1, 1A2 and 1B1)-mediated bioactivation of B[a]P was investigated using a human hepatoma cell model (HepG2). DADS and DATS inhibited the B[a]F-induced ethoxyresorufin Odeethylase (EROD) activity, a marker enzyme for CYP1, by 30-90% and 70-95% at 100-1,000μм concentration, respectively. The cell viability, an indicator of the capacity to inhibit B[a]P bioactivation, was increased by treatments of 100-1,000μм DADS and 10-100μм DATS. Immunoblot results indicated that the B[a]P inducible CYP1A2 protein was suppressed by 100-1,000μм of DADS and 10-100μм of DATS, but CYP1A1 and 1B1 were not detectable in any microsomes. Analysis of B[a]P metabolites revealed that the level of 7,8-diol formed was significantly reduced in the DADS and DATS treated microsomes as compared to the control. The level of 9,10-diol and 4,5-diol formed was also lowered by the allyl sulfide treatments. These results suggest that the protective mechanism of allyl sulfides on B[a]P-induced carcinogenesis is possibly related with the modulation of CYPl-mediated bioactivation of B[a]P.

• 社団法人日本農芸化学会の論文
• 2001-10-23

著者

• Kim H

  Dept. Of Metallugical Eng. Dong-a Univ.

• Kim Hyun

  Korea Basic Science Institute

• Choi E

  Toshiba Corp.

• CHUN Hyang

  Korea Food Research Institute

• CHOI Eun

  Korea Food Research Institute

• Chun Hyang

  Korea Food Research Institude, Sungnam, Kyonggi-do 463-746, Korea

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