Copper Reduction by Yeast Cell Wall Materials and Its Role on Copper Uptake in Debaryomyces hansenii

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Copper binding and reducing activities of cell wall materials (CWM) prepared from cells of the yeast Debaryomyces hansenii were examined. When CWM was treated with copper sulfate (0.1 mM CuSO_4), the copper was partially reduced from Cu (II) to Cu (I) and bound to CWM (below 10 nmol per mg dry wt.). The bound copper was mostly in the fraction of mannan-protein. Both copper-binding ability and protein content decreased with protease treatments. Mannan-protein prepared from CWM bound more copper than mannan did. This suggests that Cu (II) bound to the protein portion in CWM and was reduced to Cu (I). The optimum pH of copper reduction by CWM was about 5.0. The amount of copper bound to CWM increased with reducing agents and decreased with oxidizing agents. On the other hand, the copper uptake by yeast whole cells and spheroplasts was also stimulated by reducing agents, but inhibited by oxidizing agents. Furthermore, copper uptake by spheroplasts was stimulated in the presence of CWM. The optimum pH of copper uptake coincided with that of copper reducing activity. These results suggest that yeast cell wall not only supplies copper binding fields but also reduces copper, and the reduced copper is transported into yeast cells. The yeast cells may have coper-reducing proteins in the cell wall.
公益社団法人日本生物工学会の論文
1988-06-25